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. 2023 Nov 18;40(4):483–499. doi: 10.1007/s12264-023-01147-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Recombinant mMFG-E8-treated microglia promote oligodendrocyte maturation in vitro. A Primary microglia were exposed to PBS, rmMFG-E8 (200 ng/mL), myelin debris (100 μg/mL), or rmMFG-E8+myelin debris for 6 h. Then the microglia-conditioned medium (MCM) was collected and added to OPCs with differentiating medium and incubated for 2 days. OPCs and OLs are labeled by immunofluorescence staining of MBP (green), PDGRFα (red), and Olig2 (grey). Nuclei are stained with DAPI. Scale bar: 50 μm. B, C The percentage of MBP⁺Oligo2⁺ OLs (B) and MBP^-PDGFRα⁺Oligo2⁺ OPCs (C) of total Oligo2⁺ cells in PBS MCM, rmMFG-E8 MCM, myelin MCM, and myelin+rmMFG-E8 MCM treated OPCs (n = 4 per group; *P < 0.05 vs PBS MCM group, ^####P < 0.0001 vs myelin MCM group. D IGF-1 mRNA expression in primary microglia treated with PBS or rmMFG-E8 (200 ng/mL) (n = 4 per group, unpaired t-test. All data are presented as the mean ± SD.