Fig. 4. FcRn intrinsically regulate the presentation on antigen targeted to DEC205.

A WT mice were lethally irradiated and reconstituted with a mix of CD45.1⁺ WT BM and Fcgrt^−/− (CD45.2⁺) BM. After six weeks, mixed BM chimera mice were injected with anti-DEC205-OVA mAb and 20 h later, spleens were harvested and cDC1 purified. Increasing numbers of cDC1 were co-incubated with CTV-labelled OT-I or OT-II at 37 °C for 60 h or 84 h, respectively. B The number of dividing OT-I and OT-II cells was quantified by flow cytometry. Data are pooled from three independent experiments, each one done in triplicate. *P < 0.05 by by Mann-Whitney test with Holm-Sidak correction for multiple comparisons.