FIG. 3.
Northern blot analysis of vA187-Ubi RNA. RNA was extracted from SK6 cells infected either with ncp (lane 3) or cp (lane 4) vA187-Ubi. Virus-specific genomic and subgenomic RNA of positive polarity was detected by hybridization with a 32P-labelled riboprobe (6). RNAs extracted from SK6 cells freshly infected either with ncp (lane 2) or cp (lane 1) vA187-1 are shown as references. The sizes of the respective RNAs as deduced from our sequencing data (13) are 12,298 nt for wild-type genomic RNA (lanes 1 and 2), 7,534 nt for wild-type subgenomic RNA (lane 1), 12,022 nt for vA187-Ubi genomic RNA (lanes 3 and 4), and 7,762 nt for vA187-Ubi subgenomic RNA (lane 4).