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Structure of the vA187-Ubi subgenomic RNA. The deletion of the subgenomic RNA was determined by RT-PCR amplification of RNA extracted from persistently infected cells which showed a CPE when primers PR5 and CSFL001 (indicated) were used followed by direct sequencing of the PCR fragment. The vA187-Ubi subgenomic RNA differs from the corresponding RNA derived from vA187-1 (A) only by the presence of the ubiquitin sequence (B).
