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. 2024 Apr 9;14:8346. doi: 10.1038/s41598-024-59061-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Nest-building activated multiple hypothalamic regions in males. (a–v) Coronal brain sections show the distribution of c-Fos-immunoreactive cells induced by nest-building in the hypothalamus. Each dot indicates a c-Fos-immunoreactive cell. The digit indicates the distance from the bregma. Scale bar 500 μm. Enclosed areas were assessed for the density of c-Fos + cells for (w). (w) Density of c-Fos-immunoreactive cells of nest-building assay at ZT6 (MPOA, n = 9; LPOA, n = 9; PVN, n = 10; AH, n = 11; ZI, n = 8; LH, n = 9; VMH, n = 9; DMH, n = 11; ARC, n = 11; PH, n = 10) and of ad libitum at ZT6 (MPOA, n = 5; LPOA, n = 5; PVN, n = 4; AH, n = 5; ZI, n = 5; LH, n = 5; VMH, n = 5; DMH, n = 5; ARC, n = 4; PH, n = 5). Welch’s t-test. Data are shown as the mean ± s.e.m.