Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Apr 9;14:8346. doi: 10.1038/s41598-024-59061-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Excitatory and inhibitory populations of c-Fos-positive hypothalamic neurons associated with nest-building in males. (a–d) Representative microscopic images of c-Fos immunostaining with double in situ hybridization for Vglut2 and Vgat mRNA. A white arrowhead indicates a c-Fos- and Vglut2-positive cell and a yellow arrowhead indicates a c-Fos–Vgat-positive cell. A white arrow indicates a Vglut2-positive cell and a yellow arrow indicates a Vgat-positive cell. Scale bars: 10 μm. (e–j) The number of c-Fos and Vgat double-positive cells in the MPOA (e), LPOA (f), AH (g), DMH (h), LH (i), and PH (j) in the nest-building group, ad lib ZT12 group, and ad lib ZT6 group. (k–p) The number of c-Fos and Vglut2 double-positive cells in the nest-building group, ad lib ZT12 group, and ad lib ZT6 group in the MPOA (k), LPOA (l), AH (m), DMH (n), LH (o), and PH (p) (MPOA: nest-building, n = 8; ad lib ZT12, n = 5; ad lib ZT6, n = 7. LPOA: nest-building, n = 8; ad lib ZT12, n = 6; ad lib ZT6, n = 8. AH: nest-building, n = 8; ad lib ZT12, n = 6; ad lib ZT6, n = 8. DMH: nest-building, n = 5; ad lib ZT12, n = 5; ad lib ZT6, n = 7. LH: nest-building, n = 9; ad lib ZT12, n = 6; ad lib ZT6, n = 6. PH: nest-building, n = 9; ad lib ZT12, n = 6; ad lib ZT6, n = 7). Each p-value was adjusted by Holm's method after Welch's t-test. Data are shown as the mean ± s.e.m.