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Nest-building activates Esr1-negative cells in the POA. (a, b) Distribution of estrogen receptor 1 (Esr1)-positive cells and c-Fos-positive cells induced by nest-building in the POA. The red square in (b) corresponds to (c). (c) c-Fos-immunostaining with in situ hybridization for Esr1 mRNA of the LPOA. Nuclei were stained with Hoechst. Scale bars; 300 μm (a, b) and 30 μm (c).
