Figure 4.

Optimization of freezing and storage conditions to enhance the storage performance of PRP. (A) SEM images of CAG-P fibers frozen at −20°C and (B) −80°C. (C) Cumulative release of VEGF from CAG-P fibers prepared under different freezing temperatures. (D) Cumulative release of VEGF and (E) LDH at different storage temperatures. (F) Schematic representation of low-temperature inhibition of platelet anaerobic respiration. (G) Cumulative release of VEGF and (H) content of P-selectin after PRP being treated with various lyoprotectants. (I) Schematic representation of the principle of lyoprotectant protection of platelets.