TABLE 1.

Sequences of oligonucleotides used for PCR amplifications and sequencing^a

Primer, position	Sequence 5′→3′	Use
412-22f	CCTGCTGCTATGCCTCATCTTC	Sequencing
433-22r	GAAGATGAGGCATAGCAGCAGG	PCR and sequencing
477-23r	GGACAAACGGGCAACATACCTTG	PCR
685-20f	GCCATTTGTTCAGTGGTTCG	Sequencing
720-21r	GTGGGGGAAAGCCCTACGAAC	Sequencing
1276-22r	CCGCAGTATGGATCGGCAGAGG	PCR and sequencing
1303-21r	TGCGAGCAAAACAAGCGGCTA	PCR
1394-23f	CCAACTGGATCCTGCGCGGGACG	Diagnostic PCR
1425-23f	TTACGTCCCGTCGGCGCTGAATC	Diagnostic PCR
1673-24r	GAGTCCAAGAGTCCTCTTATGTAA	Diagnostic PCR
1701-20r	CCTCAAGGTCGGTCGTTGAC	Diagnostic PCR
2045-23f	TCACCTCACCATACNGCACTCAG	PCR
2694-20f	GGCATTAAACCTTATTATCC	PCR
2360-20f	CGAGGCAGGTCCCCTAGAAG	PCR
2382-21f	GAACTCCCTCGCCTCGCAGAC	PCR
2724-25f	GTTAATCATTACTTCCAAACYAGAC	PCR and sequencing
2850-22r	GTAGCTCTTGTTCCCAAGAATA	Sequencing
3100-35fb	GGCATACTACAAGCTTTGCCAGCAA ATCCGCCTCC	PCR
3207-21f	GGCCATGCAGTGGAAC/TTCCAC	Sequencing
3221-21r	TTCCACTGCATGGCCTGAGGA

^aSequences are numbered with regard to sequence hvhepb (18) starting at the EcoRI restriction site. Numbers after the dashes specify the length of the oligonucleotides; f means forward, or sense, orientation, and r means reverse, or antisense, orientation. 

^bThe HindIII restriction site is underlined.