Table 1.
Putative HuR-interacting proteins identified by LC-MS/MS ranked in order of percent occurrence by FLAG epitope found in the Contaminant Repository for Affinity Purification (CRAPome) database.
| Protein | − | + | % occurrence FLAG epitope | GO Analysis |
|---|---|---|---|---|
| ATXN2 | 0 | 13 | 0.64 | Poly(A) RNA binding and structural constituent of ribosome |
| HELZ2 | 0 | 50 | 1.28 | Poly(A) RNA binding, ligand-dependent nuclear receptor transcription coactivator activity |
| STAU2 | 0 | 16 | 1.92 | Poly(A) RNA binding, double-stranded RNA binding |
| FAM120A | 0 | 43 | 2.56 | Poly(A) RNA binding |
| TROVE2 | 0 | 18 | 2.56 | RNA binding and U2 snRNA binding. |
| EIF4G3 | 0 | 12 | 5.13 | Poly(A) RNA binding and binding |
| TRIM25 | 0 | 11 | 5.13 | Poly(A) RNA binding and acid-amino acid ligase activity. |
| AGO3 | 0 | 11 | 5.13 | Nucleic acid binding and RNA binding |
| MOV10 | 0 | 26 | 5.77 | RNA binding, helicase activity |
| ZC3HAV1 | 0 | 10 | 5.77 | Poly(A) RNA binding and NAD+ ADP-ribosyltransferase activity |
| FXR1 | 0 | 13 | 6.41 | Nucleic acid binding and RNA binding |
| HNRNPUL2 | 0 | 10 | 6.41 | Poly(A) RNA binding and kinase activity |
| STAU1 | 0 | 16 | 7.69 | Poly(A) RNA binding, double-stranded RNA binding |
| UPF1 | 0 | 45 | 8.33 | Poly(A) RNA binding |
| PRRC2C | 0 | 23 | 8.33 | Poly(A) RNA binding and protein C-terminus binding |
| FUBP3 | 0 | 16 | 8.33 | Nucleic acid binding and RNA binding |
| ATXN2L | 0 | 15 | 8.33 | Poly(A) RNA binding and protein C-terminus binding |
| MYO5A | 0 | 12 | 8.33 | Nucleic acid binding and chromatin binding |
| ELAV1 | 0 | 18 | N/A | Nucleic acid binding and RNA binding |
Columns Flag empty-vector (−) and the Flag-immunoprecipitated (+) provide the raw peptide sequences from VSMCs following IP with anti-Flag antibody. Proteins were identified by LC MS/MS.
*
Indicates Elav1 was the bait protein used to pull-down proteins for LC MS/MS. CRAPome database provided no additional information about Elav1.