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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: Andrology. 2023 Mar 21;11(7):1484–1494. doi: 10.1111/andr.13423

Figure-1: Sperm functional parameters.

Figure-1:

Cauda epididymal sperm were obtained by swim-out from mice previously exposed to control, DBP, DEHP or mixed conditions. A: Upon discarding the epididymis, the total number of sperm was count with an improved Neubauer hemocytometer. B, C, D, E: Sperm suspensions from each different conditions obtained in A were incubated in complete capacitating TYH media for 60 min and their kinematic parameters analyzed by CASA. Progressive motility (B), average path velocity (VAP) (C), curvilinear velocity (VCL) (D) and, straight linear velocity (VSL) (E) were evaluated between the different exposure groups. For VCL, VSL and VAP values indicate mean ± SEM and expressed as μ m/sec. Progressive motility is expressed as percentage out of the total motile sperm. Sperm numbers, progressive motility and VAP were analyzed using one-way ANOVA followed by Tukey’s post-hoc test. VCL and VSL data were analyzed using non-parametric Kruskal-Wallis test followed by Dunn’s post-hoc test. In all cases, these analyses were done using Graph Pad Prism software for statistical analysis with p<0.05(*), p<0.01 (**), p< 0.001 (***) (N=12).