Table 5.
Main effects | Jejunum morphology Features |
|||||
---|---|---|---|---|---|---|
PHE levels (ppm) | VH (µm) | VW (µm) | CD (µm) | VH: CD | MT (µm) | VSA (mm2) |
0 | 1,176.65 | 148.63 | 261.36 | 4.50 | 269.50 | 0.55 |
200 | 1,185.52 | 147.00 | 259.27 | 4.57 | 268.65 | 0.55 |
300 | 1,175.36 | 149.18 | 258.72 | 4.55 | 269.42 | 0.55 |
400 | 1,166.07 | 148.25 | 257.91 | 4.52 | 266.41 | 0.54 |
SEM | 15.691 | 1.313 | 1.529 | 0.072 | 1.263 | 0.009 |
Eimeria challenge | ||||||
(-) | 1,215.70a | 149.25 | 259.63 | 4.69a | 267.09b | 0.57a |
(+) | 1,136.08b | 147.21 | 259.00 | 4.39b | 269.92a | 0.53b |
SEM | 11.095 | 0.928 | 1.081 | 0.051 | 0.893 | 0.006 |
Interaction effects | ||||||
0 × (-) | 1,221.00 | 149.32 | 260.15 | 4.69 | 266.50 | 0.57 |
200 × (-) | 1,242.84 | 147.50 | 259.17 | 4.80 | 267.33 | 0.58 |
300 × (-) | 1,200.15 | 150.50 | 260.15 | 4.62 | 268.67 | 0.57 |
400 × (-) | 1,198.80 | 149.67 | 259.00 | 4.63 | 265.84 | 0.56 |
0 × (+) | 1,132.33 | 148.00 | 262.50 | 4.31 | 272.50 | 0.53 |
200 × (+) | 1,128.16 | 146.51 | 259.35 | 4.35 | 270.00 | 0.52 |
300 × (+) | 1,150.51 | 147.49 | 257.32 | 4.48 | 270.16 | 0.53 |
400 × (+) | 1,133.36 | 146.83 | 256.80 | 4.42 | 267.00 | 0.52 |
SEM | 22.190 | 1.857 | 2.162 | 0.102 | 1.786 | 0.012 |
P value | ||||||
PHE levels | 0.856 | 0.723 | 0.447 | 0.910 | 0.288 | 0.942 |
Eimeria challenge | <.0001 | 0.128 | 0.685 | 0.0002 | 0.030 | <.0001 |
PHE levels × Eimeria challenge | 0.493 | 0.928 | 0.623 | 0.419 | 0.521 | 0.844 |
Linear (PHE levels at (-)) | 0.204 | 0.659 | 0.805 | 0.380 | 0.933 | 0.557 |
Quadratic (PHE levels at (-)) | 0.540 | 0.804 | 0.971 | 0.638 | 0.306 | 0.809 |
Linear (PHE levels at (+)) | 0.825 | 0.746 | 0.055 | 0.404 | 0.059 | 0.966 |
Quadratic (PHE levels at (+)) | 0.799 | 0.810 | 0.530 | 0.660 | 0.857 | 0.923 |
Abbreviations: PHE, pistachio green hull aqueous extract; VH, villus height; VW, villus width; CD, crypt depth; VH: CD, villus height to crypt depth ratio; MT, muscle thickness; VSA, villus surface area; (-), unchallenged groups; (+), challenged groups; SEM, Standard error of the mean.
Means within a column without a common superscript significantly differ (P < 0.05).