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SDS-PAGE profiles of proteins expressed in and purified from E. coli. Lanes: A, purified His-H18E1; B, GST-H18E2; C, His-YY1; and D, GST-YY1. Proteins were purified as described in Materials and Methods, electrophoresed in SDS-polyacrylamide gels, and stained with Coomassie brilliant blue. Western blots using antibodies against the fusion tag (E1 and E2) or YY1 confirmed the identities (data not shown).
