FIG. 5.

YY1 inhibition of HPV ori replication is largely independent of binding to the ori. (A) Lack of discernible effects by the inclusion of YY1BS oligonucleotides in HPV-18 ori replication reaction mixtures containing exogenous YY1 protein. All the reactions were conducted under the optimal conditions described in the legend to Fig. 3B to replicate 40 ng of HPV-18 ori plasmid, which contains a YY1BS overlapping the E1BS, or HPV-11 ori plasmid, which does not have a known or suspected YY1BS (shown in panel B), except control reactions with a backbone plasmid (both panels, lanes 3). A double-stranded oligonucleotide containing YY1BS or mYY1BS was included in the cell-free replication reaction mixtures in the presence (+) or absence (−) of exogenous YY1. Proteins (180 ng of E1, 14 ng of E2, and 0.2 μg of YY1) or oligonucleotides (20 ng) were included as indicated. (B) All reactions were conducted under the optimal conditions described in the legend to Fig. 3B. The amounts of His-tagged YY1 added to the reactions were 0.2 μg (lane 5) and 2 μg (lane 6).