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. 1998 Jun;72(6):4911–4917. doi: 10.1128/jvi.72.6.4911-4917.1998

FIG. 7.

FIG. 7

Cotransfection of YY1 expression vectors represses HPV-18 ori replication in transient replication. (A) Repression of HPV-18 ori replication by YY1 in transfected 293 cells. Increasing amounts of pCDNA (lanes 3 to 5) or pCDNA-hYY1 (lanes 6 to 8) were cotransfected into 293 cells with expression vectors of viral E1 and E2 proteins (pMTX-H18E1 and pMTXH18E2), and the origin-containing vector (pBS-H18ori). Replicated DNA was revealed by Southern blotting after digestion with DpnI and BamHI as described in Materials and Methods. Cotransfection with pBS without viral ori (lane 1) was used as a negative control for transient replication assays. Lane M, 200 pg of linearized pBS-H18ori. (B) Repression of HPV-18 ori replication by YY1 mutated in the DNA-binding domain. An expression vector of the full-length YY1 protein (pCMV-hYY1) or of a truncated YY1 lacking the zinc finger DNA-binding domain (pCMV-hYY1DZnF) was cotransfected into 293 cells. The intensity of replication in each transfection containing YY1 expression vectors was compared to that of a control transfection. Data were collected from three independent experiments. Open bars indicate averages, and error bars indicate standard deviations.