Ong 2022.

Study name	Project Wolbachia – Singapore
Methods	Status: ongoing Aim: to determine whether large‐scale deployment of Wolbachia‐infected male Ae aegypti mosquitoes can reduce the incidence of dengue in individuals living in intervention clusters, compared to individuals living in non‐intervention clusters. Study type: cRCT Study dates: randomization February 2022, cRCT started July 2022, completion date December 2024 Country, location: Singapore Unit of allocation: community block Number of units: 15 Length of follow‐up: study duration expected to be 24 months
Participants	Number of participants: N/I ongoing Method of recruitment: N/I ongoing Loss to follow‐up: N/I ongoing Age: N/I ongoing Sex: N/I ongoing Socio‐economic status: N/I ongoing
Interventions	Wolbachia species: wAlbB Mosquito species: Ae aegypti Mosquito life stage at release: adult Strategy: population suppression Number of deployments: N/I ongoing Timing of deployments: twice per week, weekdays between 06:30 and 11:00 hours and 13:00 to 18:00 hours Location of deployments: equally spaced release locations per apartment block, on the ground, middle (levels 5 or 6), and high floors (levels 10 or 11). Aimed % vector population suppression: N/I ongoing Achieved % vector population suppression: N/I ongoing Field monitoring strategy: gavitraps. 6–9 Gravitraps per high‐rise apartment block designed to lure and trap gravid female Aedes Co‐interventions: N/I ongoing
Outcomes	Primary outcomes Dengue incidence, measured by the odds ratio of Wolbachia exposure distribution amongst laboratory‐confirmed reported dengue cases compared to test‐negative controls Prevalence of Ae aegypti/Ae albopictus mosquitoes Secondary outcome Community attitudes and acceptance
Starting date	9 September 2020
Contact information	Srilatha Edupuganti E‐mail: sedupug@emory.edu
Notes

CHIKV: chikungunya virus; cRCT: cluster‐randomized controlled trial; DENV: dengue virus; N/I: no information; ZIKV: zika virus.