Fig. 6.
Protease inhibitor profiling for affinity purified recombinant Amblyomma americanum serpin (rAAS) 41 and 46. Indicated proteases (concentration indicated in materials and methods) were incubated with affinity purified (A) rAAS41 or (B) rAAS46 (1 μM) at 37 °C for 15 min followed by addition of appropriate substrate (0.2 mM). Protease kinetics was monitored every 11 s over 15 min in triplicate.