Fig. 9.

Recombinant Amblyomma americanum serpin (rAAS)41 inhibition of chymase and α-chymotrypsin is reduced using monospecific antibody to rAAS41. In triplicate reactions, purified monospecific IgG antibodies to rAAS41 or pre-immune (PI) serum were incubated with 136 nM or 170 nM of rAAS41 at 37 °C for 30 min before the addition of 10.8 nM of chymase (A) or 12.8 nM of α-chymotrypsin (B) following an additional 15 min incubation at 37 °C. Subsequently, the chymase or α-chymotrypsin substrate (0.2 mM) were added and protease kinetics was monitored for 15 min every 15 s. Data is reported as residual enzymatic activity.