| Component | Amount (μl) | Final concentration |
|---|---|---|
| 10× dATP mix | 0.5 | 1× |
| 10× NEBuffer 2 | 2.5 | 1× |
| 5,000 U/ml Klenow fragment (3′→5′ exo-) | 1 | |
| Molecular-biology-grade water | 11 |
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