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. 2024 Apr 10;24:132. doi: 10.1186/s12935-024-03319-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — EP13 shows a rotenone-like activity. MDA-MB-231 cells were grown in 2D conditions and treated with EP13 (1, 3, 6 or 12 µM) or the complex I inhibitor, rotenone (1 µM) during a time-course (every 5 min) of OCR measurement using XFe24 Seahorse. At the end of the experiment, addition of 6 µM rotenone in both conditions completely blocked the oxygen consumption. Data are the mean ± S.D. (at least n = 3 wells per group)