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. Author manuscript; available in PMC: 2024 Apr 10.
Published in final edited form as: Science. 2023 Oct 13;382(6667):eadf0834. doi: 10.1126/science.adf0834

Fig. 2. Analysis of excitatory and inhibitory neuron lineages.

Fig. 2.

(A) Cell types, reconstructed single-cell trajectories, and age distribution for subtypes of excitatory neurons. L2–3, upper-layer intratelencephalic projection neurons; L4, layer 4 neurons; L5–6-IT, deep-layer intratelencephalic projection neurons; L6, layer 6 neurons; L5, layer 5 neurons; SP, subplate neurons. (B) Identification of interneuron trajectories. (C) Rates of maturation of subtypes of excitatory neurons and interneurons. (D) GO analysis of genes with different age of onset of expression. FDR, false discovery rate; Reg., regulation. (E) Examples of top lineage- and branch-specific genes with transient and burst expression patterns. (F) Number of transient and burst genes in specific lineages and branches. (G) Spatial transcriptomic analysis of 140 lineage-specific genes, showing the spatial map of annotated cell types across development. GW22, 22 weeks of gestation; 2wk, 2 weeks postnatal; 25yo, 25-year-old; PFC, prefrontal cortex; Ex, excitatory; radial glial. (H) Examples of deep-layer neuronal markers with early patterned layer-specific expression (putative layer location is noted in parentheses).