Fig 6.
Regulation of RusT target reporter fusions. (A–D) Regulation of target reporter fusions by RusT. C. crescentus ΔvanAB ΔrusT cells carrying the indicated gfp reporter fusion in combination with either an empty control vector (pBV-MCS6; ctrl.), or the expression plasmid pPvan-RusT were grown overnight in the presence of vanillate, and GFP expression was quantified either by fluorescence intensity measurements (A, D) or by Western blot analysis of total protein samples using RNA polymerase (RNAP) as loading control [B; quantification in (C)]. For the quantification of each GFP-fusion, expression levels in the presence of the control plasmid were set to 1, and relative changes were determined for cells expressing RusT. GFP levels were calculated from three biological replicates; error bars indicate the standard deviation.