Fig 7.
Target regulation by RusT is dependent on Hfq. (A, B) Regulation of target reporter fusions by RusT in the absence of Hfq. C. crescentus ΔvanAB ΔrusT Δhfq cells carrying the indicated gfp reporter fusion in combination with either an empty control vector (pBV-MCS6; ctrl.), or the expression plasmid pPvan-RusT were grown overnight in the presence of vanillate. Total protein samples were analyzed on Western blots to determine GFP expression; RNAP served as loading control. (B) Quantification of GFP expression as described in (A); error bars indicate the standard deviation from three biological replicates.