Fig 6.

Increased pathology in pan-Irgm^−/− mice at later timepoints in genital C. muridarum infection. Mice were infected transcervically with 2.5 × 10⁵ C. muridarum EBs and harvested at 25 dpi or 45 dpi. (A) Gross pathology of infected genital tracts. Scale bars = 0.5 in; arrowheads indicate hydrosalpinx. (B–D) Genital tracts were fixed, sectioned, and H&E stained. (B) The magnitude of acute, chronic, and total acute + chronic inflammation at 45 dpi was graded by a veterinary pathologist (wild-type n = 7 mice, pan-Irgm^−/− n = 8). Data represent one experiment that is representative of multiple replicates. (C) Low-magnification images of cystic changes in wild-type uterus and ovary/oviduct and continued inflammation in pan-Irgm^−/− uterus and ovary/oviduct. (D) Quantification of hydrosalpinx at 25 dpi and 45 dpi in genital tracts infected with C. muridarum. Data represent pooled data from three experiments (25 dpi n = 12 mice; 45 dpi wild-type n = 21, pan-Irgm^−/− n = 22). Each data point represent one uterine horn of an infected mouse. Statistical significance was determined using Mann-Whitney tests (B) or χ² test (D); *P < 0.05 and **P < 0.005; ns, not significant.