Fig 5.

Differences in STING and CD63 exocytosis between HSV-1(F) and HSV-2(G)-infected cells. (A and B) HEL cells were either uninfected or infected with HSV-1(F) or HSV-2(G) at 0.5 PFU/cell. EVs were isolated from cell supernatants at 48 h post-infection by pelleting at 100,000 × g. Total EVs were analyzed by western blot for STING, CD63, CD81, and LAMP1. Detection of STING monomers and dimers in equal amounts of cell lysates served as a control. (C) HEL cells (1 × 10⁸) were either uninfected or infected with HSV-1(F) or HSV-2(G) at 0.5 PFU/cell. Cell supernatants were harvested at 48 h post-infection and EVs were isolated through an iodixanol-sucrose gradient and analyzed by NTA as described in Materials and Methods. All values were derived after analyzing samples from three independent experiments. *P  ≤  0.05; **P  ≤  0.01; ***P  ≤  0.001. (D) HEL cells were uninfected or infected with either HSV-1(F) or HSV-2(G) at 0.5 PFU/cell. Cell lysates were harvested at 24 and 48 h post-infection. Equal amounts of proteins were analyzed by western blot for the viral proteins ICP8, gD, and VP16. B-actin was used as a loading control.