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. 2024 Jan-Mar;16(1):9–15. doi: 10.18502/ajmb.v16i1.14166

Figure 1.

Figure 1.

SDS-PAGE evaluation the total expression of E. coli BL21 (DE3) containing recombinant pTWIN1in various conditions. A) analysis of total LYC1 fusion protein expression. Lane 1: PageRuler™ unstained protein ladder 26614, Lane 2: induction by 0.1 mM IPTG at 7°C, Lane 3: induction by 0.3 mM IPTG at 7°C, Lane 4: induction by 0.5 mM IPTG at 7°C, Lane 5: induction by 0.1 mM IPTG at 22°C, Lane 6: induction by 0.3 mM IPTG at 22°C. Lane 7: induction by 0.5 mM IPTG at 22°C. Lane 8: induction by 0.1 mM IPTG at 37°C. Lane 9: induction by 0.3 mM IPTG at 37°C. Lane 10: induction by 0.5 mM IPTG at 37°C. B). analysis of total TP4 fusion protein expression. Lane 1: PageRuler™ unstained protein ladder 26614, Lane 2: induction by 0.1 mM IPTG at 7°C, Lane 3: induction by 0.3 mM IPTG at 7°C, Lane 4: induction by 0.5 mM IPTG at 7°C, Lane 5: induction by 0.1 mM IPTG at 22°C, Lane 6: induction by 0.3 mM IPTG at 22°C. Lane 7: induction by 0.5 mM IPTG at 22°C. Lane 8: induction by 0.1 mM IPTG at 37°C. Lane 9: induction by 0.3 mM IPTG at 37°C. Lane 10: induction by 0.5 mM IPTG at 37°C. C) analysis of total TP4 fusion protein expression. Lane 1: PageRuler™ unstained protein ladder 26614, Lane 2: induction by 0.1 mM IPTG at 7°C, Lane 3: induction by 0.3 mM IPTG at 7°C, Lane 4: induction by 0.5 mM IPTG at 7°C, Lane 5: induction by 0.1 mM IPTG at 22°C, Lane 6: induction by 0.3 mM IPTG at 22°C. Lane 7: induction by 0.5 mM IPTG at 22°C. Lane 8: induction by 0.1 mM IPTG at 37°C. Lane 9: induction by 0.3 mM IPTG at 37°C. Lane 10: induction by 0.5 mM IPTG at 37°C.