FIG. 2.

HIV-1-specific serum immunoglobulin subtype profiles induced by QS-21-mediated DNA vaccination via the i.m. (A) and i.n. (B) routes. The inoculation procedure and the time of blood collection were the same as those for Fig. 1. The subtype profile was determined by EIA and is presented as optical density at 450 nm. The results are expressed as means ± SEM for five or six (experimental group) or three or four (control group) mice. ∗ and #, significant enhancement or decrease in the antibody amount, respectively, compared with IIIB/REV alone (P < 0.05). Similar results were obtained in a repeat experiment (not shown).