TABLE 2.
ONT average coverage depth for (+) and (−) ssRNAs isolated from cells infected with porcine OSU strain
| RNA sequencea | Average coverage depth (reads/nucleotide) | |
|---|---|---|
| Positive strand | Negative strand | |
| gs 1 (VP1) | 3,282.45 | 196.16 |
| gs 2 (VP2) | 3,901.18 | 261.65 |
| gs 3 (VP3) | 1,223.30 | 271.19 |
| gs 4 (VP4) | 2,109.55 | 132.48 |
| gs 5 (NSP1) | 528.93 | 671.66 |
| gs 6 (VP6) | 7,705.13 | 864.61 |
| gs 7 (NSP3) | 7,465.08 | 607.89 |
| gs 8 (NSP2) | 7,987.08 | 343.86 |
| gs 9 (VP7) | 5,845.46 | 1,154.66 |
| gs 10 (NSP4) | 7,906.21 | 1,060.12 |
| gs 11 (NSP5) | 7,858.12 | 1,632.10 |
| bGAPDH | 4.12 | 0.00 |
a
Rotavirus porcine strain OSU.
b
Sequence covering GAPDH of Macaca fascicularis.