Three distinct subclasses of membrane-bound organelles are present. (A) A tomographic slice of a Plasmodium merozoite showing the rhoptries (Rh), and three distinct subclasses of membrane-bound organelles, MBO1, MBO2, and MBO3, marked with yellow, purple, and red arrows, respectively. Scale bar, 100 nm. (B) Zoomed-in views of a representative of each subclass, including a line colored as in (A) and with a black arrow marking the right edge in all cases. The red arrow indicates the electron-dense region characteristic of MBO3s. Scale bar, 50 nm. (C) Inverted pixel values of the lines shown in (B). Arrows also as in (B).