FIG. 5.

Endo H sensitivity of mutant gB molecules. Vero cell monolayers were infected with KOS (A), KgB(C633S) (B), or KgB(C596S/C633S) (C) virus at an MOI of 10. Six hours p.i., monolayers were pulse-labeled for 10 min in the presence of [³⁵S]methionine-cysteine and lysed immediately (Pulse; lanes 1 and 2) or further incubated in complete media and harvested after 1, 2, or 3 h of chase (lanes 3 and 4, 5 and 6, or 7 and 8, respectively). Samples were solubilized and subjected to immunoprecipitation with the gB-1 MAb pool, captured with protein A-Sepharose, incubated in absence (−) or presence (+) of endo H, and separated by SDS-PAGE. Molecular size markers are indicated in kilodaltons at the left.