FIG. 6.

Processing of mutant gB by glucosidases I and II. Vero cell monolayers were infected with KOS (A), KgB(C633S) (B), or KgB(C596S/C633S) (C) virus at an MOI of 10. Five hours p.i., cells were preincubated in the absence (−; lanes 1, 3, and 5) or presence (+; lanes 2, 4, and 6) of CST for 1 h. Infected cells were pulse-labeled for 10 min in the presence of [³⁵S]methionine-cysteine and chased for 2 h in absence or presence of CST. Monolayers were harvested, solubilized, and subjected to immunoprecipitation with a gB-1 MAb pool, and protein A-Sepharose immunocomplexes were separated by SDS-PAGE.