FIG. 9.

Failure of the mutant gB molecules to be transported to the cell surface in the presence of wild-type gB as detected by immunofluorescence analysis. Vero cell monolayers were individually transfected with HA-tagged gB (gBHA; row 1), gB(C633S) [gBHA(C633S); row 2], or gB(C596S/C633S) [gBHA(C596S/C633S); row 3]. Twenty-four hours posttransfection, monolayers were infected with KOS at an MOI of 5. Eight hours p.i., cells were fixed in ice-cold methanol (A) or left untreated (B and C) and incubated with anti-HA (A and C) or gB-1 MAb pool (B) antibodies followed by incubation with a cy3-conjugated anti-mouse antibody. Cells were visualized with a model 211910 Nikon microscope and photographed.