Figure 2.

Characterization of exosome enrichment performance using the functionalized SiO₂‐chip. A–F) Fluorescence imaging of the SiO₂‐chip, including a simulation in the bottom‐left. Details of experimental grouping are provided in the text under figures. G–I) 3D views of the chip. Details of experimental grouping are provided in the text under figures. J) SEM image of exosomes captured on the functionalized SiO₂‐chip K) Western blot (WB) imaging of exosomes captured by differently SiO₂‐chip, where lane 1–5 correspond to different modification methods. Grouping for lane 1–5 is consistent with B–F. L) The exosomes enrichment process of the SiO₂‐chip. Plasma was introduced into the chip and subsequently washed with PBS solution to eliminate impurities, leaving only the captured exosomes on the SiO₂‐chip. RNA lysates or RIPA lysates were passed through the chip to extract RNA or proteins from exosomes. Abbreviations: SA (streptavidin), biotin‐anti CD63 (biotinylated anti‐CD63 antibody), EXO (exosome), HCC (hepatocellular carcinoma), and PBS (phosphate‐buffered saline). Unveiling HCC‐associated lncRNAs in exosomes enriched by SiO₂‐chip through transcriptome sequencing