TABLE 1.
A-MuLV infection of CHO K1 cells transiently expressing chimeric receptors
Receptor | % Infectiona |
---|---|
Pit2 | 100 |
Pit1 | <0.1b |
GGGAA | 55.2 ± 25.4c |
GAGGG | 18.5 ± 7.4d |
GAAGG | 75.1 ± 25.3 |
GGAGG | <0.1 |
No DNA | <0.1 |
As described in Materials and Methods, data given represent the means ± the standard deviations for three (except where indicated otherwise) independent transfection-infection experiments. Each transfection precipitate was placed in triplicate wells of a 12-well dish. The average number (varying from 200 to 800 per well in different experiments) of β-galactosidase-positive foci in the wells transfected with Pit2 DNA was assigned a value of 100% for each experiment, and all other values were normalized to this.
<0.1, no β-galactosidase-positive foci were observed in three independent experiments, each done in triplicate in 12-well dishes.
Previously reported (8).
Data given represent the mean ± the standard deviation for five independent experiments.