Table 1.

Pre-TIL infusion checklist*

Cryopreserved TIL infusion product	TIL product to be thawed per manufacturer’s specification.
Fluid balance	The goal is to ensure that the patient is at baseline weight prior to infusion.
Physical examination	Weight. Gastrointestinal; cardiovascular; extremities; head; eyes, ears, nose, and throat; respiratory system; dermatologic; musculoskeletal; neurologic; and psychiatric examinations. Baseline ophthalmology exam in patients with a history of uveitis from prior therapy to ensure complete resolution.
Vital signs	Pulse rate, O2 saturation, respiratory rate, blood pressure, and temperature.
Blood tests	Hematology: CBC with differentials (WBC with differentials, RBC, hemoglobin, hematocrit, MCV, MCH, platelet count). Blood chemistry: Sodium, potassium, chloride, total carbon dioxide or bicarbonate, creatinine, glucose, BUN, albumin, calcium total, magnesium total, phosphorus, alkaline phosphatase, ALT/SGPT, AST/SGOT, total bilirubin, direct bilirubin, LDH, total protein, and uric acid. Inflammatory markers: CRP and ferritin.
Line placement	Central venous catheter/PICC line.
Premedication	Acetaminophen or equivalent. Diphenhydramine or other H1-histamine antagonist.
Medications available at bedside during infusion	Continued acetaminophen (650 mg q4h) or equivalent. Indomethacin (50–75 mg q6h) or equivalent. Pantoprazole (40 mg) or famotidine (20 mg) or equivalent. Meperidine (25–50 mg) and/or hydromorphone. Epinephrine and diphenhydramine. Other medications as per institutional standards.
Other concurrent medications	All other concurrent medications to be stopped during TIL infusion.

*Pre-non-myeloablative lymphodepletion checklist is provided in online supplemental table 3.

ALT, alanine aminotransferase; AST, aspartate aminotransferase; BUN, blood urea nitrogen; CBC, complete blood count; CRP, C reactive protein; IV, intravenous; LDH, lactate dehydrogenase; MCH, mean corpuscular hemoglobin; MCV, mean corpuscular volume; NSAID, non-steroidal anti-inflammatory drug; PICC, peripherally inserted central catheter; q4h, every 4 hours; q6h, every 6 hours; RBC, red blood cell count; SGOT, serum glutamic oxaloacetic transaminase; SGPT, serum glutamate pyruvate transaminase; TIL, tumor-infiltrating lymphocyte; WBC, white blood cell count.