Figure 3.

Macrophage NAMPT drives TAM polarity toward M2‐like phenotypes. A) mRNA of Arg‐1, Nos2 and Ifn‐β were quantified in WT and Nampt KO macrophages co‐cultured with MC38 cells for 12 h. mRNA levels of the indicated genes were normalized to the mRNA level of Tbp1. B‐D) BMDMs were directly co‐cultured with tumor cells for the indicated times (B) and were co‐cultured with tumor cells using a transwell system for the indicated times (C). MC38 tumor‐conditioned medium was used as <3‐kDa fractions to stimulate macrophages for the indicated times (D). Representative flow cytometry plots (left) and bar graph (right). Results are represented as the mean ± SEM. Statistical analysis was performed using the unpaired two‐tailed Student's t‐test.