FIG. 1.

Detection of desmoplakin and nuclear expression of CMV IE or pp65 antigens in trophoblast cultures by double immunohistochemical staining. Column-purified villous CT were induced to syncytialize by the addition of 10 ng of EGF per ml and challenged on day 5 of culture with CMV strain AD169 at an MOI of 1.0. At day 12 postinfection, cultures were immunohistochemically stained for CMV antigens with Ni-DAB substrate and for desmoplakin with AEC substrate. (A) Infected culture stained for CMV IE antigen and desmoplakin; (B) infected culture stained for pp65 and desmoplakin; (C) infected culture stained for desmoplakin. Bar, 25 μm.