KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| E. coli cells DH10Bac | Invitrogen | 10361012 |
| Chemicals, peptides, and recombinant proteins | ||
| Benzamidine | Sigma-Aldrich | Cat# B6506 |
| Leupeptin | Sigma-Aldrich | Cat# L2884 |
| Lauryl Maltose Neopentyl Glycol (LMNG) | Anatrace | Cat# NG310 |
| Glyco-diosgenin (GDN) | Anatrace | Cat# GDN101 |
| Cholesterol hemisucinate TRIS Salt (CHS) | Sigma-Aldrich | Cat# C6512 |
| Sodium Cholate | Sigma-Aldrich | Cat# C1254 |
| TCEP | Thermo Fisher Scientific | Cat# 77720 |
| Apyrase | NEB | Cat# M0398S |
| Ethylenediamine Tetraacetic Acid (EDTA) | Thermo Fisher Scientific | Cat# S311-500 |
| Monoclonal ANTI-FLAG M2-FITC antibody | Sigma- Aldrich | Cat# F4049 |
| Fenoldopam | Med Chem Express | Cat# HY-B0735A |
| A77636 | TOCRIS | Cat# 1701 |
| Dopamine | Selleck | Cat# S2529 |
| SKF83959 | TOCRIS | Cat# 2074 |
| PW0464 | This paper | N/A |
| LY3154207 | This paper | N/A |
| [3H]-SCH23390 | PerkinElmer | NET930250UC |
| Critical commercial assays | ||
| HTRF Dynamic cAMP kit | Cisbio | Cat# 62AM4PEB |
| Q5 site directed mutagenesis kit | NEB | Cat# E0554S |
| cAMP Glosensor Assay | Promega | Cat# E1291 |
| Deposited data | ||
| Fenoldopam-DRD1-Gs EM map and coordinate | This paper | EMDB: EMD-30392 PDB: 7CKW |
| A77636-DRD1-Gs EM map and coordinate | This paper | EMDB: EMD-30393 PDB: 7CKX |
| PW0464-DRD1-Gs EM map and coordinate | This paper | EMDB: EMD-30394 PDB: 7CKY |
| Dopamine-PAM-DRD1-Gs EM map and coordinate | This paper | EMDB: EMD-30395 PDB: 7CKZ |
| SKF83959-DRD1-Gs EM map and coordinate | This paper | EMDB: EMD-30452 PDB: 7CRH |
| Experimental models: cell lines | ||
| Insect cell line Sf9 | Expression Systems | N/A |
| HEK293 cell | ATCC | N/A |
| Primers for site-direct mutagenesis | ||
| DRD1-S198A_forward: catctcaGCCtctgtaataagcttttacatccctg | This paper | N/A |
| DRD1-S198A_reverse: attacagaGGCtgagatggcatatgtcctgctga | This paper | N/A |
| DRD1-S202A_forward: CtgtaataGCCttttacatccctgtggccatcatg | This paper | N/A |
| DRD1-S202A_reverse: tgtaaaaGGCtattacagaggatgagatggcatat | This paper | N/A |
| DRD1-D103A_forward: cctttGCCatcatgtgctccactgcatccatc | This paper | N/A |
| DRD1-D103A_reverse: agcacatgatGGCaaaggccacccagatgttacagaag | This paper | N/A |
| Recombinant DNA | ||
| pFastBac1-HA-FLAG-DRD1-His*10 | This paper | N/A |
| pcDNA3.1-HA-FLAG-DRD1 (WT and mutants) | This paper | N/A |
| pFastBac1-Gαs | This paper | N/A |
| pFastBac1Dual-Gβγ | This paper | N/A |
| Software and algorithms | ||
| SerialEM | Mastronarde DN | https://bio3d.colorado.edu/SerialEM/ |
| MotionCor2 | (Zheng et al., 2017) | https://emcore.ucsf.edu/ucsf-software |
| Gctf | Zhang, 2016 | https://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/ |
| Gautomatch | Zhang, 2016 | https://www.mrc-lmb.cam.ac.uk/kzhang/Gautomatch |
| Relion3.0 | (Zivanov et al., 2018) | https://www2.mrc-lmb.cam.ac.uk/relion |
| CHIMERA | Pettersen et al., 2004 | http://www.cgl.ucsf.edu/chimera |
| COOT | (Emsley and Cowtan, 2004) | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| Phenix | Adams et al., 2010 | https://www.phenix-online.org |
| PyMOL | Schrodinger | https://www.pymol.org/2/ |
| Prism v.7.0 | GraphPad Software | https://www.graphpad.com |
| Other | ||
| Superdex 200 Increase column 10/300 | GE healthcare | Cat# 28990944 |
| BIOTEK Cytation3 reader | BIO-TEK | N/A |
| ESF921 cell culture medium | Expression Systems | Cat# 96-001 |
| DMEM basic cell culture media | GIBCO | Cat# 11995 |
| FBS (fetal bovine serum) | Cell Box | Cat# SAG-01U-02 |
| Penicillin-Streptomycin | GIBCO | Cat# 15140122 |
| Trypsin 0.5% EDTA | GIBCO | Cat# 25300054 |
| 9 cm2 Cell Culture Dish | Corning | Cat# 430166 |
| 6-well Cell Culture Dish | Corning | Cat# 430166 |
| 96-well Cell Culture Dish | Corning | Cat# 3599 |
| 96-well white microplate assay plates | Corning | Cat# 3917 |