Skip to main content
. 2024 Apr 10;132(4):047005. doi: 10.1289/EHP13435

Figure 5.

Figures 5A to 5D are volcano plots titled 2 milligrams per week polystyrene, 4 milligrams per week polystyrene, 2 milligrams per week mixed plastics, and 4 milligrams per week mixed plastics, plotting negative log 10 (lowercase p), ranging from 0 to 6 in increments of 2; 0 to 3 in unit increments; 0 to 4 in unit increments; and 0 to 5 in unit increments (y-axis) across log 2 (fold change), ranging from negative 2 to 2 in increments of 2; negative 2 to 3 in unit increments; negative 4 to 2 in increments of 2; and negative 5.0 to 5.0 in increments of 2.5 (x-axis) for status, including down, non-sig, and up, respectively. Figure 5E is a set of two Venn diagrams tiled Exposure comparison. On the left, the Venn diagram displays two circles. The left circle is labelled 2 milligrams per week polystyrene with 128 metabolites, the right circle is labelled 4 milligrams per week polystyrene with 16 metabolites. The intersection area displays 43 metabolites. On the right, the Venn diagram displays two circles. The left circle is labelled 2 milligrams per week mixed plastics with 20 metabolites, the right circle is labelled 4 milligrams per week mixed plastics with 44 metabolites. The intersection area has 73 metabolites.

Untargeted metabolomics of liver. Untargeted metabolomic analysis in the liver of mice exposed to (A) 2mg/week polystyrene, (B) 4mg/week polystyrene, (C) 2mg/week mixed plastics, or (D) 4mg/week mixed plastics. Data plotted as log(2) fold difference (p<0.05). (E) Venn diagram representing the significantly different metabolites following microplastic exposures (p<0.05 as compared to control). Mice were exposed twice a week for weeks with 5-μm polystyrene microspheres or mixed polymers [polystyrene, polyethylene, and poly-(lactic-co-glycolic acid)] at 2mg/week (low dose) or 4mg/week (high dose); n=8 per group. Source data can be found in Excel Tables S5 and S6.