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. Author manuscript; available in PMC: 2024 Apr 10.
Published in final edited form as: Mol Biochem Parasitol. 2020 Sep 20;240:111317. doi: 10.1016/j.molbiopara.2020.111317

Figure 6: AD12 epitope is not removed by PNGase F or O-glycosidase.

Figure 6:

(A) The Wb-CFA was treated with PNGase F one hour at 37°C then analyzed by AD12 western blot. (B) AD12 western blot of 0.5μg B. malayi lysate or ES after a one-hour PNGase F treatment. (C) AD12 western blot of Wb-CFA following one-hour incubation with a deglycosidase mix containing O-glycosidase, PNGase F, Neuraminidase, β1–2 galactosidase, and N-acetylglucosaminidase under native or denaturing conditions. (D) AD12 western blot of B. malayi lysate and ES after a one-hour denaturing deglycosidase reaction. A corresponding silver stain of each reaction is available in supplementary figure S1 that includes the reaction positive controls RNase B for PNGase F and fetuin for deglycosidase mix.