Fig. 3. Stability and function of IgG heterodimers.

(A to F) First derivative plots of the melting temperatures of the Fab₁Fc forms of each of the indicated constructs. The labeled temperatures are the mean of six total replicates, three for each orientation in which the mutations per chain are on the Fc fragment only, or on the Fc and Fab fragment. (G) K_D values for WT IgG1, combYSelect1, and combYSelect2 IgG derived from BLI binding assays. Statistical significance was determined by one-way ANOVA with Tukey’s multiple-comparison test (ns P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001).