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. 2024 Mar 21;151(6):dev201621. doi: 10.1242/dev.201621

Fig. 3.

Fig. 3.

Transcriptome analysis of human pluripotent stem cells cultured on soft or hard gel, or in a conventional glass dish. (A) Hierarchical clustering analysis of mRNA expression in human pluripotent stem cells (hPSCs) cultured on soft and hard substrates compared with conventional glass dishes (n=2). The figure displays the mean value of the two samples. (B) A volcano plot representing the entire gene expression dataset for hPSCs cultured on a soft gel versus dish for 4 days. Red color indicates differentially expressed genes; dots represent individual genes. Insignificant genes are in gray. (C) Expression levels of selected genes based on RNA-seq of hPSCs cultured on soft or hard gels, or in a conventional glass dish. Expression level is shown as transcripts per kilobase million (TPM). (D) Endoderm-related gene expression based on RNA-seq of hPSCs cultured on soft or hard gel, or in a conventional glass dish. Expression level is shown as TPM. Data are mean±s.d.