Figure 6.

Administration of MB during aging does not affect grip strength or alter skeletal expression of antioxidant enzymes. Eighteen-months old female C57BL/6J were divided into two groups. One group was exposed to regular drinking water and the other to 250 μM MB-containing water for 6 months. (A) Grip strength of the front paws (FP) or all four paws (AP) were determined at 18 (basal) and 24 months using the NBP20-16 Grip Strength apparatus. Data presented as a mean value of three consecutive measurements for FP and AP. 18 months-old females n = 48, 24 months-old females n = 13, 24 months-old BM-treated females n = 16. (B–H) Gene expression was tested in tibia cortical bone by real-time PCR of 18 months old (basal) and 24 months old mice.- including PGC1a-peroxisome proliferator-activated receptor-g coactivator-1a, ATP6-mitochondrial ATP synthase 6, Cox1-cytochrome oxidase subunit 1, MnSOD2-manganase-dependent superoxide dismutase, Catalase 1, Catalase 2, and GPX1- glutathione peroxidase 1. Data tested by multivariate ANOVA and presented as mean ± SEM of n = 5. Significance accepted at p < 0.05 (^*p < 0.05, ^**p < 0.01, ^***p < 0.01, ^****p < 0.0001).