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Southern blot analysis of strain 293 Hygr clones derived from Bac-ITR/RepA infection. Conditions of infection and DNA analysis were as described in Fig. 6. (A) Hybridization to aavs1 probe. (B) Same membrane hybridized to transgene probe. The upshifted bands that are annealed to both probes are considered to be indicative of site-specific integration and are indicated with arrows. The positions of the molecular size standards (in kilobases) are indicated.
