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In situ hybridization of metaphase chromosomes from a pool of 400 strain 293 Hygr clones derived from Bac-ITR/RepA infection. Chromosome preparations were hybridized with a Hyg–β-Gal-specific (in red) or aavs1-specific (in yellow) probe as described in Materials and Methods. (A) The transgene is integrated into a chromosome other than chromosome 19. (B) Colocalization of the transgene and of the aavs1 probe.
