FIG. 9.

(A) PCR amplification of the ITR-aavs1 junction in baculovirus-infected strain 293 cells. Cells were infected at the indicated MOI and were collected 4 days postinfection; total DNA was prepared and subjected to nested PCR amplification as described in Materials and Methods. Bands corresponding to primers and amplified junctions are indicated. (B) ITR-aavs1 junction sequences from Bac-ITR/RepA-infected 293 and MRC-5 cells. Common bases at the ITR-aavs1 junction are underlined. Sequences not belonging to ITR or aavs1 are in boldface. X62488 was identified in GenBank with a Blast search. Sequences are depicted in ITR, aavs1 order.