KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
ADGRE2-CAR (anti-idiotype, 23D5.G5.D4.C10, Dylight405)	This paper	N/A
CLEC12A-CCR (anti-idiotype, 18F11.E11.C6, PE)	This paper	N/A
human EGFRt (AY13, BV711)	Biolegend	Cat# 352920; RRID: AB_2687123
human CD3 (UCHT1, BUV615)	BD Biosciences	Cat# 612992; RRID: AB_2870263
human CD4 (L200, BUV395)	BD Biosciences	Cat# 564107; RRID: AB_2738596
human CD8 (SK1, BUV805)	BD Biosciences	Cat# 612889; RRID: AB_2833078
human CD14 (63D3, SparkBlue550)	Biolegend	Cat# 367148; RRID: AB_2832724
human CD16 (CB16, SuperBright600)	ThermoFisher	Cat# 63-0168-42; RRID: AB_2662479
human CD19 (SJ25C1, BUV563)	BD Biosciences	Cat# 612916; RRID: AB_2870201
human CD25 (BC96, PE)	Biolegend	Cat# 302606; RRID: AB_314276
human CD34 (581, APC/Fire750)	Biolegend	Cat# 343536; RRID: AB_2650736
human CD38 (HB-7, BV650)	Biolegend	Cat# 356620; RRID: AB_2566233
human CD45 (HI30, PacificOrange)	ThermoFisher	Cat# MHCD4530; RRID: AB_10376143
murine CD45 (30-F11, AF488)	Biolegend	Cat# 103122; RRID: AB_493531
human CD45RA (HI100, BV480)	BD Biosciences	Cat# 566114; RRID: AB_2739516
human CD71 (M-A712, BV711)	BD Biosciences	Cat# 563767; RRID: AB_2738413
human CD90 (5E10, PE/Dazzle594)	Biolegend	Cat# 328134; RRID: AB_2566343
human CD33 (P67.6, PE/Cy7)	Biolegend	Cat# 366618; RRID: AB_2566420
human CD123 (6H6, BV785)	Biolegend	Cat# 306032; RRID: AB_2566448
human ADGRE2 (2A1, AF647)	Bio-Rad	Cat# MCA2330A647T; RRID: AB_2231142
human CLEC12A (50C1, BB515)	BD Biosciences	Cat# 565325; RRID: AB_2739182
human IFNg (B27, PE)	Biolegend	Cat# 506507; RRID: AB_315440
human IFNg (blocking)	Bio X Cell	Cat# BE0235; RRID: AB_2687717
Biological samples
Mobilized Leukopaks®, G-CSF x 5 Days - Apheresis Day 6, Fresh	Miltenyi Biotec	Cat# 150-000-461
Normal donor whole bone marrow, fresh	Allcells	N/A
Buffy coats	New York Blood Center	N/A
AML patient samples	MSKCC	IRB# 06-107 and IRB# 14-091
AML patient-derived xenograft models	MSKCC, Antitumor Assessment Core Facility	IRB# 14-091
Chemicals, peptides, and recombinant proteins
Cas9-NLS purified protein	QB3-Berkeley Macrolab	N/A
P3 Primary Cell 4D-Nucleofector® X Kit	Lonza	Cat# V4XP-3024
Human IL-7, premium grade	Miltenyi Biotec	Cat# 130-095-363
Human IL-15, premium grade	Miltenyi Biotec	Cat# 130-095-765
Dynabeads™ CD3	ThermoFisher	Cat# 11151D
Critical commercial assays
Quantum™ Simply Cellular® antiMouse IgG	Bangs Laboratories	Cat# 815
Pan T Cell Isolation Kit, human	Miltenyi Biotec	Cat# 130-096-535
Deposited data
Normal tissue scRNAseq data	Human Protein Atlas (v21.1)	RRID: SCR_006710; Download:https://www.proteinatlas.org/download/rna_single_cell_type_tissue.tsv.zip
Experimental models: Cell lines
Phoenix-AMPHO	ATCC	Cat# CRL-3213; RRID: CVCL_H716
EL4	ATCC	Cat# TIB-39; RRID: CVCL_0255
MOLM-13	DSMZ	Cat# ACC 554
U-937	ATCC	Cat# CRL-1593.2; RRID: CVCL_0007
Experimental models: Organisms/strains
NSG mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ	Jackson Laboratory	RRID: IMSR_JAX:005557
NSG-SGM3 mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1 Eav/MloySzJ	Jackson Laboratory	RRID: IMSR_JAX:013062
Oligonucleotides
TRAC sgRNA target sequence: 5′-CAGGGTTCTGGATATCTGT	Synthego	N/A
IFNG sgRNA target sequence: 5′-CCAGAGCATCCAAAAGAGTG	Synthego	N/A
Recombinant DNA
pCMV-gag-pol	Cell Biolabs	Cat# RV-111
pCMV-VSV-G	Cell Biolabs	Cat# RV-110
pSFG-ADCLEC.syn1	This paper	N/A
pSFG-ADGRE2-28z1XX-P2A-EGFRt	This paper	N/A
pSFG-CLEC12A-BB-P2A-EGFRt	This paper	N/A
pSFG-ADGRE2-28z1XX-P2A-CLEC12A-del	This paper	N/A
pSFG-CD33-28z 1XX-P2A-EGFRt	This paper	N/A
pSFG-CD19-28z1XX-P2A-EGFRt	This paper	N/A
Software and algorithms
GraphPad Prism v10.0.1	GraphPad Software	N/A
FlowJo v10.8.1	BD Biosciences	N/A
Living Image	Perkin Elmer	N/A
Adobe Illustrator v27.8.1	Adobe	N/A