Table 1.

Sequence featured of the synthetic locus

		Synthetic locus
	Feature	HPRT1	HPRT1R	HPRT1RnoCpG
	Length	100,667	100,667	95,067
	GC content	0.41	0.41	0.38
Dinucleotides	AA	6,601	6,601	6,601
	AC	4,942	6,952	5,803
	AG	7,012	5,734	6,883
	AT	7,491	6,759	6,759
	CA	6,952	4,942	5,743
	CC	4,261	4,261	3,442
	CG	1,202	4,499	0
	CT	7,038	5,751	6,583
	GA	5,734	7,012	6,211
	GC	4,499	1,202	726
	GG	4,348	4,348	4,455
	GT	5,368	7,388	6,556
	TA	6,759	7,491	7,491
	TC	5,751	7,038	5,797
	TG	7,388	5,368	6,609
	TT	7,415	7,415	7,415
CpG	Expected	4,291	4,291	3,386
	Ratio	0.28	1.05	0
	Yeast TFBSsa	5,159	18,191	13,284
Mouse TFBSsb	p-val < 0.001	24,578	19,782	21,897
	q-val < 0.01	1,132	707	882

^aYeast TFBSs as predicted using the YEASTRACT+ database⁶⁵.

^bMouse TFBSs as predicted using FIMO⁶⁶ in the MEME suite with the JASPAR vertebrate motif database⁶⁷.