Fig. 4. Fna C2 impact on intestinal tumorigenesis and metabolism.
a, A schematic of the study with 6–8-week-old ApcMin+/− mice receiving streptomycin and dextran sodium sulfate (DSS) treatment to alter the native microbiome and induce colitis, respectively. Mice were orally gavaged with vehicle control (arm 1) or a mix of three representative Fna C1 (arm 2) and Fna C2 (arm 3) strains. A strain mix was used to capture a higher proportion of Fna clade-specific accessory genes (Extended Data Fig. 2d). The mice were monitored until the end-point at 6 weeks post-gavage when they reached 15–17 weeks of age. b, A plot indicating the number of adenomas in the large intestine by treatment arm (vehicle control (grey); Fna C1 treated (green); Fna C2 treated (lavender); n = 8 mice per arm). The data are plotted as mean ± s.e.m. The statistical analysis was carried out using one-way ANOVA. c, Partial least squares discriminant analysis of detected intestinal metabolites (n = 1,296). The colours represent the treatment arm (vehicle control (grey); Fna C1 treated (green); Fna C2 treated (lavender)). The graphics in a were created using BioRender.com.