Fig. 6.

Effect of E. bonariensis on OVX/D-Gal-induced changes in hippocampal expression of A α7-nAChR, B p-Jak2, and C p-STAT3. Rats underwent either SO or OVX, and after 5 days they received D-Gal (150 mg/kg/day, i.p) for 42 days. OVX/D-Gal-subjected rats were orally treated with donepezil (5 mg/kg/day) or the alcoholic extract of E. bonariensis (100 mg/kg/day) for 42 days, given 1 h prior to D-Gal. One day after behavioral testing (day 43), rats were decapitated, and hippocampi were separated for biochemical analysis. Data were expressed as mean ± SD (n = 5 for α7-nAChR gene expression, while n = 3 for p-Jak2 and p-STAT3 protein expression), using one-way ANOVA followed by Tukey's post hoc test (P < 0.05). OVX: ovariectomy, D-Gal: D-galactose, Jak2: Janus kinase 2, SO; sham operation, STAT3: signal transducer and activator of transcription 3